Affiliation:
1. Carotenoid Research Group, School of Biological and Earth Sciences, Liverpool John Moores University, Byrom Street, Liverpool L3 3AF, United Kingdom.
Abstract
Abstract
We examined the effect of irradiance on the synthesis of β-carotene and its isomers by Dunaliella salina. Growth irradiance had a marked effect both on growth of the alga (which was suppressed at both low and high irradiances) and on the accumulation of β-carotene. The accumulation of β-carotene but not α-carotene was closely linked to an increase in irradiance. Growth at low irradiances (20–50 μmol m−2s−1) promoted a high ratio of 9-cis to all-trans β-carotene (>2:1), while exposure to high irradiances (200–1,250 μmol m−2 s−1) resulted in a large reduction in this ratio (to <0.45:1). A similar pattern was seen for the geometric isomers of α-carotene, with exposure to low irradiance favoring the accumulation of the 9-cis form. The carotenoid biosynthesis inhibitors 4-chloro-5(methylamino)-2-(α-α-α-trifluoro-m-tolyl)-3-(sH)-pyridazinone and 2-(4-chlorophenylthio)triethylamine caused the accumulation of the precursors phytoene and lycopene, respectively, in D. salina. High-performance liquid chromatography and infrared analysis showed that phytoene adopted the 15-cis and all-trans forms (as in higher plants), and that lycopene primarily adopted the all-trans form. This indicates that isomerization of β-carotene takes place during or after cyclization.
Publisher
Oxford University Press (OUP)
Subject
Plant Science,Genetics,Physiology
Cited by
37 articles.
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