γ-Glutamyl Transpeptidase in Transgenic Tobacco Plants. Cellular Localization, Processing, and Biochemical Properties

Author:

Storozhenko Sergei1,Belles-Boix Enric1,Babiychuk Elena1,Hérouart Didier2,Davey Mark W.1,Slooten Luit3,Van Montagu Marc1,Inzé Dirk1,Kushnir Sergei1

Affiliation:

1. Vakgroep Moleculaire Genetica, Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, K.L. Ledeganckstraat 35, B–9000 Gent, Belgium (S.S., E.B.-B., E.B., M.W.D., M.V.M., D.I., S.K.);

2. Laboratoire de Biologie Végétale et Microbiologie, Université de Nice, F–06108 Nice cedex 2, France (D.H.); and

3. Laboratorium voor Biofysica, Vrije Universiteit Brussel, B–1050 Brussels, Belgium (L.S.)

Abstract

Abstract γ-Glutamyl transpeptidase (γ-GT) is a ubiquitous enzyme that catalyzes the first step of glutathione (GSH) degradation in the γ-glutamyl cycle in mammals. A cDNA encoding an Arabidopsis homolog for γ-GT was overexpressed in tobacco (Nicotiana tabacum) plants. A high level of the membrane-bound γ-GT activity was localized outside the cell in transgenic plants. The overproduced enzyme was characterized by a high affinity to GSH and was cleaved post-translationally in two unequal subunits. Thus, Arabidopsis γ-GT is similar to the mammalian enzymes in enzymatic properties, post-translational processing, and cellular localization, suggesting analogous biological functions as a key enzyme in the catabolism of GSH.

Publisher

Oxford University Press (OUP)

Subject

Plant Science,Genetics,Physiology

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