Affiliation:
1. Department of Quality Assurance, Shri. D.D. Vispute College of Pharmacy and Research Center, Devad Vichumbe, Panvel 410221, Maharashtra, India
Abstract
The well-known tropical tree Psidium guajava, also known as the guava, is widely farmed for its fruit. This article consists of literature about various analytical techniques like TLC, UV spectrophotometry, HPLC, and HPTLC that could be used to analyze the Guava plant. The HPLC analysis of Quercetin isolated from the leaves of the guava plant uses a 4 x 125 mm Hypersil ODS column with a mobile phase of 0.5 percent ortho-phosphoric acid in water and methanol at a flow rate of 1ml/min. The column wash times are 20 min and 18 min, respectively. 250C is the temperature in the column. Silica gel 60F 254 pre-coated plates (10x10 cm) were used with toluene: acetone: formic acid (38:10:5) as the solvent system for HPTLC analysis of quercetin obtained from the tender leaves of guava. The λ max was observed in the U.V. spectrum of the isolated compound (quercetin) on three standard wavelengths: 212 nm, 256 nm, and 372 nm. An effort can be made to produce a new analytical method that will be cost-effective and time-saving.
Reference44 articles.
1. Wani MS, Polshettiwar SA, Parakh SR, Chopade VV. Herbal Medicine and its Standardization. Pharm Rev. (2007) 5(6):1â14.
2. Naseer S, Hussain S, Naeem N, Pervaiz M, Rahman M. The phytochemistry and medicinal value of Psidium guajava (guava). Clin Phytosci. (2018) 4(32):1â8.
3. Joseph B, Mini Priya R. Review on nutritional medicinal and pharmacological properties of Guava. Int J Pharm Sci. (2011) 2(1):53â64.
4. Shirur DS, Adhikari R, Sharma ST, Sajjekhan S. A review on the medicinal plant Psidium Guajava Linn (MYRTACEAE). J Drug Deliv Ther. (2013) 3(2):162â168.
5. Begum S, Hassan, SI, Ali SN, Siddiqui BS. Chemical constituents from the leaves of Psidium guajava. Nat Prod Res. (2004) 18(2):135â140.