Estimation of Paraproteins by Immunoturbidimetry and Electrophoresis Followed by Scanning Densitometry

Author:

Sinclair David1,Ballantyne Fiona1,Shanley Susan1,Caine Eleanor1,O'Reilly Denis1,Shenkin Alan1

Affiliation:

1. Institute of Biochemistry. Royal Infirmary, Glasgow G4 OSF, UK

Abstract

A comparison of paraprotein estimation by immunoturbidimetry (IT) and electrophoresis followed by scanning densitometry (EFD) showed significant differences in concentration for IgG, IgA, and IgM paraproteins. Overestimation of IgM paraproteins by IT was particularly marked. Our data suggest that both techniques should be used for IgG and IgA paraproteins but for IgM paraproteins IT was found to be unsuitable and EFD is the method of choice.

Publisher

SAGE Publications

Subject

Clinical Biochemistry,General Medicine

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