Use of Touch-Down Polymerase Chain Reaction to Enhance the Sensitivity of Mycobacterium Bovis Detection-Reference-Cited by-同舟云学术

Use of Touch-Down Polymerase Chain Reaction to Enhance the Sensitivity of Mycobacterium Bovis Detection

Published:2005-05 Issue:3 Volume:17 Page:232-238
ISSN:1040-6387
Container-title:Journal of Veterinary Diagnostic Investigation
language:en
Short-container-title:J VET Diagn Invest

Author:

Zumárraga Martín J.¹,Meikle Virginia¹,Bernardelli Amelia²,Abdala Alejandro³,Tarabla Hector³,Romano María I.¹,Cataldi Angel¹

Affiliation:

1. Instituto de Biotecnología Rafaela, 2300 Argentina.

2. CICVyA/INTA, Los Reseros y las Cabañas, 1712 Castelar, Argentina, DILACOT-SENASA Rafaela, 2300 Argentina.

3. Martinez, and the EEA Rafaela 2300 Argentina.

Abstract

The confirmatory diagnosis of Mycobacterium bovis ( M. bovis) in animal samples is carried out by culture in Stonebrink media. However, culture is very slow because of the extremely long duplication time of the bacillus and difficult because of the scarcity of bacilli in diagnostic samples. This study describes the development of a single-tube touch-down polymerase chain reaction (PCR) protocol for the detection of M. bovis using primers that target the IS 6110 element. Spiked water and milk as well as routine diagnostic samples (milk and nasal swabs) from M. bovis–positive cattle were tested. This protocol allows the rapid and sensitive detection of M. bovis in bovine samples by enhancing the sensitivity of standard PCR amplification.

Publisher

SAGE Publications

Subject

General Veterinary

Link

http://journals.sagepub.com/doi/pdf/10.1177/104063870501700303

Reference37 articles.

1. A One-Tube Nested Polymerase Chain Reaction for the Detection of Mycobacterium Bovis in Spiked Milk Samples: An Evaluation of Concentration and Lytic Techniques

2. Large-scale use of polymerase chain reaction for detection of Mycobacterium tuberculosis in a routine mycobacteriology laboratory

3. Identification of an insertion sequence, IS1081, inMycobacterium bovis

4. Comparison of C 18 -Carboxypropylbetaine and Glass Bead DNA Extraction Methods for Detection of Mycobacterium bovis in Bovine Milk Samples and Analysis of Samples by PCR

5. Tuberculosis in seals caused by a novel member of the Mycobacterium tuberculosis complex: Mycobacterium pinnipedii sp. nov.

Cited by 45 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Effects of primers, PCR approaches and sample preservation methods on diversity studies of myxobacteria;Journal of Microbiological Methods;2023-08

2. Genomic comparison of two strains of Mycobacterium avium subsp. paratuberculosis with contrasting pathogenic phenotype;Tuberculosis;2023-01

3. Short Communication: Prevalence of Listeria monocytogenes in Raw Milk of Healthy Sheep and Goats;Veterinary Medicine International;2022-05-19

4. Mycobacterium tuberculosis infection in a free-ranging urban dog from Argentina;Veterinary Research Communications;2022-02-08

5. Prevalence of Mycobacterium bovis in milk on dairy cattle farms: An international systematic literature review and meta-analysis;Tuberculosis;2022-01