Comparison of C 18 -Carboxypropylbetaine and Glass Bead DNA Extraction Methods for Detection of Mycobacterium bovis in Bovine Milk Samples and Analysis of Samples by PCR-Reference-Cited by-同舟云学术

Comparison of C 18 -Carboxypropylbetaine and Glass Bead DNA Extraction Methods for Detection of Mycobacterium bovis in Bovine Milk Samples and Analysis of Samples by PCR

Published:1998-08 Issue:8 Volume:64 Page:3099-3101
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Cornejo Brandon J.¹,Sahagún-Ruiz Alfredo²,Suárez-Güemes Francisco²,Thornton Charles G.³,Ficht Thomas A.⁴,Adams L. Garry⁴

Affiliation:

1. Department of Biology, Colorado State University, Fort Collins, Colorado 805231;

2. Departamento de Microbiologı́a Inmunologı́a, Facultad de Medicina Veterinaria, Universidad Nacional Autónoma de México, México, Distrito Federal 04510, México2;

3. Department of Molecular Biology & Genetics, Quest Diagnostics Incorporated, Baltimore, Maryland 212273; and

4. Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, Texas 77843-44674

Abstract

ABSTRACT The purpose of this prospective study was to compare two different milk preparation methods to assay for the presence of Mycobacterium bovis by PCR. Detection by a C 18 -carboxypropylbetaine (CB-18)-based sample processing method was compared to extraction of DNA from milk with glass beads. Samples from 17 skin test-positive cattle were analyzed. Following CB-18 processing and glass bead extraction, the sensitivity of IS 6110 -based PCR was 94.1 and 58.8%, respectively ( P < 0.025). Because CB-18 processing will permit the proficient use of PCR for diagnosis and surveillance of bovine tuberculosis, it will contribute to the more efficient detection and control of tuberculosis.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.64.8.3099-3101.1998

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