Fibroblast cell proliferation on photo-cured trehalose cinnamoyl ester thin films

Abstract

Trehalose cinnamoyl esters (TCs) were synthesized by esterification of trehlaose with cinnamoyl chloride. Two TCs with different degrees of substitution were synthesized, and trehalose cinnamoyl ester thin film-coated glass plates were prepared by a dip-coating method. Photocuring of the TCs was confirmed by ultraviolet–visible spectral changes. The surface of photo-cured TCs were found smooth as observed in a scanning electron microscope. The cell proliferation on the photo-cured TCs was investigated using 3T3 Swiss Albino mouse embryo fibroblasts. The results of the cell proliferation assay revealed that the photo-cured TCs with higher degrees of substitution promoted the cell proliferation compared with a polystyrene culture plate and the photo-cured TCs with lower degrees of substitution. The contact angle of the photo-cured TCs with higher degrees of substitution was 101.0° ± 1.6°, which is much higher than that of the polystyrene culture plate, and it is out of the range known to be suitable for cell adhesion. Nevertheless, the cell unexpectedly grew best on the photo-cured TCs with higher degrees of substitution. Fibronectin binding assay was carried out using fluorescent probe-modified fibronectin, and more fibronectin was found adsorbed onto photo-cured TCs than a polystyrene culture plate.