DENV-2 Outbreak Associated With Cosmopolitan Genotype Emergence in Western Brazilian Amazon-Reference-Cited by-同舟云学术

DENV-2 Outbreak Associated With Cosmopolitan Genotype Emergence in Western Brazilian Amazon

Published:2024-01 Issue: Volume:18 Page:
ISSN:1177-9322
Container-title:Bioinformatics and Biology Insights
language:en
Short-container-title:Bioinform Biol Insights

Author:

Ribeiro Jessiane Rodrigues¹²,Roca Tárcio Peixoto²³,Cartonilho Gleense dos Santos¹²⁴,Passos-Silva Ana Maísa¹²⁴^ORCID,Moreira Hillquias Monteiro¹²⁴,Teixeira Karolaine Santos¹²,da Silva André Luiz Ferreira¹²⁴,Lugtenburg Celina Aparecida Bertoni⁵,dos Santos Alcione Oliveira⁵,Villalobos Salcedo Juan Miguel⁶,do Nascimento Valdinete Alves⁷,de Souza Victor Costa⁷,Roque Rosemary Aparecida⁸,Krieger Marco Aurélio⁹,Naveca Felipe Gomes⁷,Rampazzo Rita de Cássia Pontello⁹,Vieira Deusilene Souza¹²⁴

Affiliation:

1. Laboratório de Virologia Molecular, Fundação Oswaldo Cruz Rondônia—Fiocruz Rondônia (FIOCRUZ/RO), Porto Velho, Brazil

2. Instituto Nacional de Ciência e Tecnologia de Epidemiologia da Amazônia Ocidental—INCT-EpiAmO, Porto Velho, Brazil

3. Programa de Pós-Graduação em Medicina Tropical, Instituto Oswaldo Cruz/IOC, FIOCRUZ, Rio de Janeiro, Brazil

4. Programa de Pós-Graduação em Biologia Experimental, Universidade Federal de Rondônia—UNIR/FIOCRUZ/RO, Porto Velho, Brazil

5. Laboratório Central de Saúde Pública de Rondônia—LACEN/RO, 76.803-620, Porto Velho, Brazil

6. Centro de Pesquisa em Medicina Tropical—CEPEM, Porto Velho, Brazil

7. Instituto Leônidas & Maria Deane—Fundação Oswaldo Cruz do Amazonas, ILMD, Manaus, Brazil

8. Instituto Nacional de Pesquisas na Amazônia, INPA, Manaus, Brazil

9. Instituto Carlos Chagas, ICC, Fundação Oswaldo Cruz—Paraná, Curitiba, Brazil

Abstract

Background: Dengue virus (DENV) causes an important disease and directly affects public health, being the arbovirus that presents the highest number of infections and deaths in the Western Brazilian Amazon. This virus is divided into 4 serotypes that have already circulated in the region. Methodology: Molecular characterization of a cohort containing 841 samples collected from febrile patients between 2021 and 2023 was analyzed using a commercial kit to detect the main arboviruses circulating in Brazil: Zika, DENV-1, DENV-2, DENV-3, DENV-4 and, Chikungunya. Subsequently, Sanger sequencing was performed for positive samples. Results: The cohort detected 162 positive samples, 12 for DENV-1 and 150 identified as DENV-2, indicating co-circulation of serotypes. The samples were subjected to sequencing and the analysis of the sequences that obtained good quality revealed that 5 samples belonged to the V genotype of DENV-1 and 46 were characterized as DENV-2 Cosmopolitan genotype—lineage 5. Conclusion: The results allowed us to identify for the first time the Cosmopolitan genotype in Rondônia, Brazilian Western Amazon, and its fast spread dispersion.

Funder

Instituto Nacional de Ciência e Tecnologia de Epidemiologia da Amazônia Ocidental - INCT EpiAmO

Fundação Rondônia de Amparo ao Desenvolvimento das Ações Científicas e Tecnológicas e à Pesquisa do Estado de Rondônia - FAPERO

Publisher

SAGE Publications

Link

https://journals.sagepub.com/doi/pdf/10.1177/11779322241251581

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