Affiliation:
1. Environmental Health Sciences Program, School of Public Health, University of Massachusetts, Amherst, Massachusetts 01003, USA
Abstract
In vitro inhibition studies with β-glucuronidase from purified E. coli and mouse intestinal contents indicated that the polymer, poly(vinylbenzyl D-glucaro(1,4)lactonate, is an effective β-glucuronidase inhibitor. Purified E. coli β-glucuronidase was inhibited by 99.6% with 1 77 mM D-glucaro(1,4)lactone using the polymer-inhibitor. Similarly, 95% inhibition of β-glucuronidase activity of mouse intestinal contents was obtained with 177 mM and 50% inhibition was obtained with 31.5 mM D-glucaro(1,4)lactone based on the modified polymer. The structural requirements of an effective β-glucuronidase inhibitor based on the structure of the polymer-inhibitor are also discussed.
Subject
Health, Toxicology and Mutagenesis,Toxicology,General Medicine
Cited by
5 articles.
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