Prototype Instruments for Laboratory and On-Line Measurement of Lipoxygenase Activity

Abstract

A laboratory bench-top and a continuous prototype for measurement of lipoxygenase activity was developed. Lipoxygenase activity determination was based on the measurement of the rate of oxygen consumption by the enzyme using a polarographic sensor for the bench-top prototype and fibre optic sensors for the continuous prototype. The bench-top and the continuous sensors were compared against the standard spectrophotometric method. The range of LOX activity according to the spectrophotometric assay was from 0 to 0.163 μmol/L s. Accuracy and precision were 0.009 and 0.005 μmol/L s respectively for the bench top prototype and 0.007 and 0.006 μmol/L s for the on-line prototype. Correlation coefficients ( R2) were 0.99 for both prototypes with respect to the spectrophotometric method.