Biotin and Digoxigenin as Labels for Light and Electron Microscopy in Situ Hybridization Probes: Where Do We Stand?-Reference-Cited by-同舟云学术

Biotin and Digoxigenin as Labels for Light and Electron Microscopy in Situ Hybridization Probes: Where Do We Stand?

Published:1997-04 Issue:4 Volume:45 Page:481-491
ISSN:0022-1554
Container-title:Journal of Histochemistry & Cytochemistry
language:en
Short-container-title:J Histochem Cytochem.

Author:

Chevalier Jacques¹,Yi Jing²,Michel Odile¹,Tang Xue-Ming²

Affiliation:

1. Unité de Recherche “Immunopathologie Humaine”, INSERM U430, Hôpital Broussais, Paris, France (JC,OM)

2. Department of Biophysics and Cell Biology, Shanghai Second Medical University, Shanghai, China (JY,XMT)

Abstract

Biotin was recently applied to detect cellular DNA or RNA. In combination with avidin, streptavidin or antibody, it can be conjugated with fluorescent dye, enzyme, ferritin, or gold. However, emphasis has recently been placed on the false-positive results that are obtained when this probe is used, because endogenous biotin may sometimes interfere with specific signals. Digoxigenin appears to be an interesting alternative because it is present exclusively in Digitalis plants as a secondary metabolite. We discuss in this review the efficiency and the respective advantages and disavantages of these two probes for in situ hybridization, mainly at the electron microscopic level.

Publisher

SAGE Publications

Subject

Histology,Anatomy

Link

http://journals.sagepub.com/doi/pdf/10.1177/002215549704500401

Reference72 articles.

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