Digoxigenin-Labeled In Situ Hybridization for the Detection of Streptococcus suis DNA in Polyserositis and a Comparison with Biotinylated In Situ Hybridization

Author:

KANG Ikjae1,SEO Hwi Won1,PARK Changhoon1,OH Yeonsu1,LEE Jeehoon2,YOU Ok Heui3,KIM Sung-Hoon3,GOTTSCHALK Marcelo4,CHAE Chanhee1

Affiliation:

1. Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151–742, Republic of Korea

2. Dong Bang Co., Ltd., Dogok-Dong 517–8, Gangam-gu, Seoul 135–272, Republic of Korea

3. College of Oriental Medicine, Kyunghee University, 1 Hoegi-dong, Dongdaemun-Gu, Seoul 130–701, Republic of Korea

4. Groupe de Recherche sur les Maladies Infectieuses du Porc and Centre de Recherche en Infectiologie Porcine, Faculty of Veterinary Medicine, University of Montreal, Quebec, Canada

Publisher

Japanese Society of Veterinary Science

Subject

General Veterinary

Reference19 articles.

1. 1. Aragon, V., Segales, J. and Oliveira, S. 2012. Glasser’s disease. pp. 760–769. In: Diseases of Swine, 10th ed. (Zimmerman, J. J., Karriker, L. A., Ramirez, A., Schwartz, K. J. and Stevenson, G. W. eds.), Blackwell Publishing, West Sussex, U.K.

2. 2. Brown, C. C., Baker, D. C. and Baker, I. K. 2007. Alimentary system. pp. 288–289. In: Pathology of Domestic Animals, 5th ed. (Maxie, M. R. ed.), Saunders, College Publishing, Philadelphia, Pennsylvania.

3. 3. Chatellier, S., Harel, J., Zhang, Y., Gottschalk, M., Higgins, R., Devriese, L. A. and Brousseau, R. 1998. Phylogenetic diversity of Streptococcus suis strains of various serotypes as revealed by 16S rRNA gene sequence comparison. Int. J. Syst. Bacteriol. 48: 581–589.

4. 4. Chevalier, J., Yi, J., Michel, O. and Tang, X. M. 1997. Biotin and digoxigenin as labels for light and electron miscroscopy in situ hybridization probes: where do we stand?J. Histochem. Cytochem. 45: 481–491.

5. 5. Cooper, K. M., Kennedy, S., McConnell, S. and Kennedy, D. G. 1997. An immunohistochemical study of the distribution of biotin in tissues of pigs and chickens. Res. Vet. Sci. 63: 219–225.

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