Development of a bead-based suspension array for the detection of pathogens in acute respiratory tract infections-Reference-Cited by-同舟云学术

Development of a bead-based suspension array for the detection of pathogens in acute respiratory tract infections

Published:2016-07-24 Issue:14 Volume:241 Page:1551-1558
ISSN:1535-3702
Container-title:Experimental Biology and Medicine
language:en
Short-container-title:Exp Biol Med (Maywood)

Author:

Chen Yu-Sheng¹,Li Hong-Ru¹,Zhang Wei¹,Hua Zhi-Dan¹,Lin Xiao-Hong¹,Lin Meng-Qing¹,Huang Wen-Sen¹,Huang Li-Ping¹,Yu Xiao-Li¹,Xu Neng-Luan¹,Lin Ming¹,Xie Bao-Song¹,Shen Xiao-Na²,Xie Jian-Feng²,Wang Yi³,Huang Meng²,Wu Yan-An⁴,Hu Xin-Lan⁴

Affiliation:

1. Department of Respiratory and Critical Care Medicine, Fujian Provicial Hospital, Fujian Provincial Medical College, Fujian Medical University, Fuzhou 350001, China

2. Department of Viral Diseases, Fujian Center for Disease Control and Prevention, Fuzhou 350001, China

3. Shanghai Tellgen Life Science Co. Ltd, Shanghai 201203,China

4. Clinical Microbiology Laboratory, Fujian Provincial Hospital, Fuzhou 350001, China

Abstract

We developed a high-throughput bead-based suspension array for simultaneous detection of 20 respiratory tract pathogens in clinical specimens. Pathogen-specific genes were amplified and hybridized to probes coupled to carboxyl-encoded microspheres. Fluorescence intensities generated via the binding of phycoerythrin-conjugated streptavidin with biotin-labeled targets were measured by the Luminex 100 bead-based suspension array system. The bead-based suspension array detected bacteria in a significantly higher number of samples compared to the conventional culture. There was no significant difference in the detection rate of atypical pathogensatypical pathogens or viruses between the bead-based suspension array and real-time PCR. This technology can play a significant role in screening patients with pneumonia.

Publisher

SAGE Publications

Subject

General Biochemistry, Genetics and Molecular Biology

Link

http://journals.sagepub.com/doi/pdf/10.1177/1535370216647128

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