Numerical modeling of the effects hydration and number of hydrogen bonds on the mechanical properties of the tropocollagen molecule

Abstract

Bone aging involves structural and molecular modifications, especially at the level of type I tropocollagen. This macromolecule shows two main age-related alterations, which are the decrease of both molecular diameter (due to the loss of hydration) and number of hydrogen bonds. In this work, it is proposed to investigate the influence of these two parameters (molecular diameter and number of hydrogen bonds) on the mechanical behavior of tropocollagen using finite element method. To this end, a novel three-dimensional finite element model of collagen molecule accounting for hydrogen bonds was developed. Then, a numerical design of experiments for the diameter of tropocollagen and variations in the number of hydrogen bonds has been established. The mechanical properties (“load–strain” curve and apparent Young’s modulus) of the collagen molecule were obtained by employing the proposed model to uniaxial tensile tests. The parametric study demonstrates that the mechanical properties of tropocollagen are slightly affected by the rate of hydration but considerably affected by variation of the number of hydrogen bonds. Finally, a fitted analytical function was deduced from the above results showing effects of the two parameters (hydration rate and hydrogen bonds) on the apparent Young’s modulus of tropocollagen. This study could be useful to understand the influence of structural age modifications of tropocollagen on the macroscopic mechanical properties of bone.