Affiliation:
1. Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, China
2. Anning First People's Hospital Affiliated to Kunming University of Science and Technology, kunming, China
Abstract
Objective/Background: Cancer is one of the leading causes of death in the world. Therefore, there is an urgent need to develop new drugs for fighting this disease. Recent studies have reported that antimicrobial peptides (AMPs) can be used as anticancer drugs. Methods: We discovered brevinin-1EG, a novel antimicrobial peptide from Pelophylax nigromaculatus, using gene cloning techniques. Brevinin-1EG was then investigated for its structure and biological activity. Results: Brevinin-1EG, with an alpha-helical structure, showed broad-spectrum anti-proliferative activity against several cancer cell lines, including U-2OS, HepG2, HT-29, A375, HeLa, and A549 cells, with IC50 values ranging from 15 to 22 μg/mL. These same concentrations were insufficient to induce hemolysis in erythrocytes, indicating the safety of this molecule. Brevinin-1EG also exhibited antioxidant activity, scavenging ABTS·+ and DPPH free radicals. Conclusion: Brevinin-1EG has good safety and potential use as an anticancer therapeutic agent. It provides support for the research of new peptide drugs and templates for structural modification.
Funder
Yunnan Ten Thousand Talents Plan Young & Elite Talents Project and Major Science
National Natural Science Foundation of China
Subject
Complementary and alternative medicine,Plant Science,Drug Discovery,Pharmacology,General Medicine