Preparative Separation of Four Major Bufadienolides from the Chinese Traditional Medicine, Chansu, Using High-Speed Counter-Current Chromatography-Reference-Cited by-同舟云学术

Preparative Separation of Four Major Bufadienolides from the Chinese Traditional Medicine, Chansu, Using High-Speed Counter-Current Chromatography

Published:2010-07 Issue:7 Volume:5 Page:1934578X1000500
ISSN:1934-578X
Container-title:Natural Product Communications
language:en
Short-container-title:Natural Product Communications

Author:

Xin Xiu Lan¹,Liu Junying¹,Ma Xiao Chi²,Wei Qing¹,Lv Li²,Wang Chang Yuan²,Yao Ji Hong²,Cui Jian³

Affiliation:

1. Biotechnology Application Center, Beijing Vocational College of Electronic Science and Technology, Beijing, P. R. China

2. School of Pharmaceutical Sciences, Dalian Medical University, Dalian 116044, P. R. China

3. Center University for Nationalities, Chinese Minority Traditional Medical Center, Beijing 10081, P. R. China

Abstract

A preparative, high-speed, counter-current chromatographic (HSCCC) method for the isolation and purification of bufadienolides from Chansu was successfully developed by using stepwise elution with a two-phase solvent system composed of n-hexane: chloroform: methanol: water (4:1:2.5:5 and 4:1:4:5, v/v). A total of 7.5 mg of cinobufotalin (1), 8.0 mg of bufalin (2), 14.0 mg of cinobufagin (3) and 9.5 mg of resibufogenin (4) were obtained in a one-step separation from 80 mg of the crude extract with purities of 93.2%, 98.7%, 99.2%, and 99.4%, respectively. The chemical structures were determined from 1H NMR and 13C NMR spectroscopic data.

Publisher

SAGE Publications

Subject

Complementary and alternative medicine,Plant Science,Drug Discovery,Pharmacology,General Medicine

Link

http://journals.sagepub.com/doi/pdf/10.1177/1934578X1000500709

Reference19 articles.

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3. Activity of Drugs from Traditional Chinese Medicine toward Sensitive and MDR1- or MRP1-Overexpressing Multidrug-Resistant Human CCRF-CEM Leukemia Cells

4. QSAR Evaluation of the Ch'an Su and Related Bufadienolides against the Colchicine-Resistant Primary Liver Carcinoma Cell Line PLC/PRF/5

5. Effects of bufalin and cinobufagin on the proliferation of androgen dependent and independent prostate cancer cells

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