Simultaneous Quantification of Echinacea Species, Flos Lonicerae, Radix Scutellaria and Fructus Forsythiae Combinations by Rapid Resolution Liquid Chromatography

Author:

Ma Jie123,Ma Yuan-Chun12,Cai Carina12,Wang Daniel12,Hou Fei Fei1,Luo Mai1,Lu Shane1,Gorecki Dariusz C.3,Patel Asmita V.3,Chen Alice1,Jin Peter1

Affiliation:

1. Canadian Phytopharmaceuticals Corp., 12233 Riverside Way, Richmond, B.C., Canada V6W 1K8

2. Canadian Institute of Medicinal Plants, 12233 Riverside Way, Richmond, B.C., Canada V6W 1K8

3. School of Pharmacy and Biomedical Sciences, University of Portsmouth, St. Michael's Building, White Swan Road, Portsmouth, PO1 2DT, UK

Abstract

Echinacea angustifolia and E. purpurea are commonly used in North America for their antibacterial effects. Flos Lonicerae, Radix Scutellaria and Fructus Forsythiae are traditional Chinese medicinal herbs commonly used for the treatment of complaints such as pneumonia, acute upper respiratory tract infection, and acute bronchitis. A reproducible, simple, and reliable rapid resolution liquid chromatographic (RRLC) method has been developed to analyze extracts of products formulated containing E. angustifolia, E. purpurea, Flos Lonicerae, Radix Scutellariae and Fructus Forsythiae simultaneously in one run in less than 6 minutes. The method uses a C18-HST column, a mobile phase consisting of 0.1% aqueous phosphoric acid solution and acetonitrile, and UV detection at 327 nm and 229 nm. A stability test was performed that revealed that chlorogenic acid is more stable in acidic pH, and hence it is best to keep the extract of E. augustifolia, E. purpurea, Flos Lonicerae, Radix Scutellariae and Fructus Forsythiae in mild acidic conditions at approximately pH 5.

Publisher

SAGE Publications

Subject

Complementary and alternative medicine,Plant Science,Drug Discovery,Pharmacology,General Medicine

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