Cytotoxicity of 9,11-Dehydroergosterol Peroxide Isolated from Ganoderma Lucidum and its Target-related Proteins-Reference-Cited by-同舟云学术

Cytotoxicity of 9,11-Dehydroergosterol Peroxide Isolated from Ganoderma Lucidum and its Target-related Proteins

Published:2010-08 Issue:8 Volume:5 Page:1934578X1000500
ISSN:1934-578X
Container-title:Natural Product Communications
language:en
Short-container-title:Natural Product Communications

Author:

Cui Ya-Jun¹,Guan Shu-Hong²,Feng Li-Xing²,Song Xiao-Yi¹,Ma Chao²,Cheng Chun-Ru²,Wang Wen-Bo²,Wu Wan-Ying²,Yue Qing-Xi²,Liu Xuan²,Guo De-An²

Affiliation:

1. Shanghai University of Chinese Traditional Medicine, Shanghai 201203, P.R. China

2. Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, P.R. China

Abstract

The cytotoxicty of 9,11-dehydroergosterol peroxide (DHEP) isolated from the fruiting bodies of Ganoderma lucidum on HeLa cells was studied. DHEP treatment for 48 h inhibited the proliferation of HeLa human cervical carcinoma cells with an IC50-value of 8.58 ± 0.98 μM. Morphological changes of DHEP-treated cells indicated that DHEP induced apoptosis in HeLa cells. To identify the cellular targets of DHEP, two-dimensional electrophoresis analysis was performed to compare the protein expression profiles of DHEP-treated cells with that of control cells. Proteins altered in expressional level after DHEP exposure were identified by MALDI-TOF MS/MS. The cytotoxic effect of DHEP was associated with regulated expression of 6 proteins. Stathmin 1 might be an important target-related protein of DHEP. The regulation of stathmin 1 by DHEP treatment was also confirmed by Western blotting.

Publisher

SAGE Publications

Subject

Complementary and alternative medicine,Plant Science,Drug Discovery,Pharmacology,General Medicine

Link

http://journals.sagepub.com/doi/pdf/10.1177/1934578X1000500806

Reference20 articles.

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