Changes in the Concentrations of Deoxynucleotides in Human Lymphocytes TreatedIn Vitrowith Genotoxic Agents-Reference-Cited by-同舟云学术

Changes in the Concentrations of Deoxynucleotides in Human Lymphocytes TreatedIn Vitrowith Genotoxic Agents

Published:1991-02 Issue:1 Volume:19 Page:26-29
ISSN:0261-1929
Container-title:Alternatives to Laboratory Animals
language:en
Short-container-title:Altern Lab Anim

Author:

Ferraro Paola¹,Biasin Maria R.¹,Celotti Lucia¹,Bianchi Vera¹

Affiliation:

1. Department of Biology, University of Padova, via Trieste 75, 35121 Padova, Italy

Abstract

We studied the toxic and genotoxic effects of UV light and an alkylating agent (methyl methanesulphonate; MMS) in human peripheral blood lymphocytes (PBL) in Goand after PHA stimulation. We measured DNA repair synthesis and replication in treated PBL together with deoxynucleoside triphosphate pool and ATP concentrations. In addition to its genotoxic action, MMS induced a strong toxic effect in cycling cells, as shown by a marked depletion of nucleotide pools.

Publisher

SAGE Publications

Subject

Medical Laboratory Technology,Toxicology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

http://journals.sagepub.com/doi/pdf/10.1177/026119299101900106

Reference13 articles.

1. The role of deoxynucleoside triphosphate pools in the inhibition of DNA-excision repair and replication in human cells by hydroxyurea

2. Inhibitors of ribonucleotide reductase alter DNA repair in human fibroblasts through specific depletion of purine deoxynucleotide triphosphates

3. Mutagenesis and deoxyribonucleotide pool imbalance

4. UV irradiation alters deoxynucleoside triphosphate pools in Escherichia coli

5. Rapid changes in deoxynucleoside triphosphate pools in mammalian cells treated with mutagens

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