Preparation and Validated Analysis of Anthocyanin Concentrate from the Calyces of Hibiscus sabdariffa-Reference-Cited by-同舟云学术

Preparation and Validated Analysis of Anthocyanin Concentrate from the Calyces of Hibiscus sabdariffa

Published:2017-01 Issue:1 Volume:12 Page:1934578X1701200
ISSN:1934-578X
Container-title:Natural Product Communications
language:en
Short-container-title:Natural Product Communications

Author:

Opletal Lubomir¹,Chocholousova-Havlikova Lucie²,Siatka Tomas¹,Cahliková Lucie¹,Locarek Miroslav¹,Ali Badreldin H.³,Manoj Priyadarshini³,Ramkumar Aishwarya³,Al Suleimani Yousuf M.³,Al Za'abi Mohammed³,Karaca Turan⁴,Nemmar Abderrahim⁵

Affiliation:

1. ADINACO Research Group, Faculty of Pharmacy, Charles University, Heyrovskeho 1203, 500 05 Hradec Králové, Czech Republic

2. Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Heyrovskeho 1203, 500 05 Hradec Králové, Czech Republic

3. Department of Pharmacology and Clinical Pharmacy, College of Medicine and Health Sciences, Sultan Qaboos University, Al Khod, Oman

4. Department of Histology- Embryology, Faculty of Medicine, University of Trakya, Balkan Campus, 22030, Edirne, Turkey

5. Department of Physiology, College of Medicine and Health Sciences, United Arab Emirates University, PO Box 17666, Al Ain, UAE

Abstract

Extracts of Hibiscus sabdariffa calyces have been shown to have various medicinal properties, some of which have been reported to be due to anthocyanins present in the calyces. To study whether these claims are valid, it is necessary to produce an extract with a high anthocyanin content and to have available a method to identify and quantify the individual compounds in the product. A method of extraction and purification has been developed based on a polyamide column chromatographic purification step. Using this method, anthocyanin concentrates were produced containing from 57 to 64% of delphinidin-3-sambubioside plus cyanidin-3-sambubioside. A rapid, efficient and validated HPLC analytical method was developed and used for the analysis of the anthocyanin concentrate.

Publisher

SAGE Publications

Subject

Complementary and alternative medicine,Plant Science,Drug Discovery,Pharmacology,General Medicine

Link

http://journals.sagepub.com/doi/pdf/10.1177/1934578X1701200112

Reference25 articles.

1. Phytochemical, pharmacological and toxicological aspects ofHibiscus sabdariffa L.: a review

2. Long-term ingestion of Hibiscus sabdariffa calyx extract enhances myocardial capillarization in the spontaneously hypertensive rat

3. Protective effect of Hibiscus anthocyanins against tert-butyl hydroperoxide-induced hepatic toxicity in rats

4. Inhibition of angiotensin convertin enzyme (ACE) activity by the anthocyanins delphinidin- and cyanidin-3-O-sambubiosides from Hibiscus sabdariffa

5. Effect ofHibiscus anthocyanins-rich extract induces apoptosis of proliferating smooth muscle cell via activation of P38 MAPK and p53 pathway

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