Simple and robust LC–MS/MS analysis method for therapeutic drug monitoring of micafungin

Abstract

Aim: To develop a simple and robust LC–MS/MS method to quantify concentrations of micafungin in human plasma for pharmacokinetic studies and therapeutic drug monitoring. Methods: Sample preparation involved protein precipitation with acetonitrile:methanol (83:17% v/v) and [13C6]-micafungin as internal standard. A rapid and selective method for micafungin was validated across a range of 0.200–10.0 mg/l. Results: The calculated accuracy for the eight-point calibration ranged from 0.7 to 5.3%. Within-run precision ranged from 0.8 to 5.9%, between-run precision ranged from 0.7 to 3.1%, and overall precision ranged from 1.3 to 6.6%. Conclusion: A simple and robust LC–MS/MS method for analyzing micafungin in human plasma has been validated and was utilized for quantification of micafungin.