Validated UHPLC–MS/MS method for quantification of doxycycline in abdominal aortic aneurysm patients

Abstract

Aim: There is a strong evidence that doxycycline can benefit abdominal aortic aneurysms patients because of its ability to inhibit matrix metalloproteinase enzymes. There is a need for a specific quantification method for doxycycline in these patients. We report herein the development and validation of a selective, specific, simple and rapid UHPLC–MS/MS method for doxycycline. Results: The assay was linear (r2 > 0.994) over a concentration range of 0.010–7.005 μg/ml. This validated method has good lower limit of quantification (LLOQ) (10 ng/ml), accuracies (89.1–103.4%), precision (≤7.0%) and extraction recoveries (95.8%). Doxycycline was stable during short- and long-term storage. Conclusion: The assay is reliable and has been successfully applied to serum samples obtained from the patients of N-TA3CT clinical study.