CRISPR-Cas13a-based diagnostic method for Chlamydia trachomatis from nongonococcal urethritis-Reference-Cited by-同舟云学术

CRISPR-Cas13a-based diagnostic method for Chlamydia trachomatis from nongonococcal urethritis

Published:2021-06 Issue:11 Volume:13 Page:901-912
ISSN:1757-6180
Container-title:Bioanalysis
language:en
Short-container-title:Bioanalysis

Author:

Huang Kaichen¹²,Yu Hailing³,Chen Zhenhua¹²,Lin Guanfeng¹²,Zhang Zhigao¹²,Zhang Xiaoyan⁴,Dong Ying¹²,Chen Hong⁴,Zhang Zhen⁴,Ma Weifeng⁵,Wu Yingsong¹,Liu Tiancai¹²⁶^ORCID

Affiliation:

1. Institute of Antibody Engineering, School of Laboratory Medicine & Biotechnology, Southern Medical University, Guangzhou, 510515, Guangdong, PR China

2. Key Laboratory of Antibody Engineering of Guangdong Higher Education Institutes, Southern Medical University, Guangzhou, 510515, Guangdong, PR China

3. Guangdong Provincial Key Laboratory of Biomedical Imaging & Guangdong Provincial Engineering Research Center of Molecular Imaging, The Fifth Affiliated Hospital, Sun Yat-Sen University, Zhuhai, Guangdong Province, 519000, PR China

4. Department of Endocrinology, Zhujiang Hospital, Southern Medical University, 253, Gongyedadao Middle, Guangzhou, 510282, Guangdong, PR China

5. Department of Microbiology, School of Public Health, Southern Medical University, Guangzhou, 510515, Guangdong, PR China

6. Guangdong Provincial Key Laboratory of Construction & Detection in Tissue Engineering, Southern Medical University, Guangzhou, 510515, Guangdong, PR China

Abstract

Aim: Development of a routine screening technique for Chlamydia trachomatis infection. The proposed approach involves the CRISPR RNA (crRNA). In the presence of the target sequence, the RNase activity of the Cas13a protein is activated, and it cleaves RNA fluorescent probe so that fluorescence will be emitted. Results: The sensitivity of the detection based on CRISPR-Cas13a was 10 fM. The results obtained by CRISPR-Cas13a and quantitative polymerase chain reaction were closely correlated: χ2 = 81.798 (p < 0.001). Conclusion: The method can be carried out at room temperature and yields results within 2 h. The developed technique does not require expensive instruments and, thus, can meet the needs of community hospitals and other institutions for screening.

Funder

The Major National Science and Technology Project of China

The National High Technology Research and Development Program (“863” program) of China

The National Natural Science Foundation of China

Publisher

Future Science Ltd

Subject

Medical Laboratory Technology,Clinical Biochemistry,General Pharmacology, Toxicology and Pharmaceutics,General Medicine,Analytical Chemistry

Link

https://www.future-science.com/doi/pdf/10.4155/bio-2021-0022

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