Determination of propranolol and carvedilol in urine samples using a magnetic polyamide composite and LC–MS/MS

Abstract

Aim: β-blockers are compounds that bind with adrenoreceptors hindering their interaction with adrenalin and noradrenalin. They are clinically relevant and they are also used in some sport as doping agents. Results: A new method based on the combination of dispersive micro-solid phase extraction and LC–MS/MS has been developed to determine propranolol and carvedilol in urine samples. For this purpose a magnetic-polyamide composite is synthesized and used as sorbent. Working under the optimum conditions, the method provides limits of detection and quantification in the range of 0.1–0.15 μg/l and 0.3–0.5 μg/l, for carvedilol and propranolol, respectively. The precision, expressed as RSD, was better than 9.6% and the relative recoveries varied between 73.7 and 81.3%. Conclusion: The methodology is appropriate for the determination of β-blockers in urine samples at the low microgram per liter range for therapeutic purposes.