Metabolic profile of human coelomic fluid-Reference-Cited by-同舟云学术

Metabolic profile of human coelomic fluid

Published:2017-01 Issue:1 Volume:9 Page:37-51
ISSN:1757-6180
Container-title:Bioanalysis
language:en
Short-container-title:Bioanalysis

Author:

Virgiliou C¹,Valianou L¹,Witting M²³,Moritz F²,Fotakis C⁴,Zoumpoulakis P⁴,Chatziioannou A C¹,Lazaros L⁵,Makrydimas G⁵,Chatzimeletiou K⁶,Raikos N⁷,Theodoridis GA¹

Affiliation:

1. Department of Chemistry, Aristotle University, Thessaloniki, Greece

2. Research Unit Analytical BioGeoChemistry Helmholtz Zentrum Munchen, German, Research Center for Health and Environment Neuherberg, Germany

3. Chair of Analytical Foodchemistry, Wissenschaftszentrum Weihenstephan für Ernährung, Landnutzunng und Umwelt, Technische Universität München

4. Institute of Biology, Medicinal Chemistry & Biotechnology, National Hellenic Research Foundation, Athens, Greece

5. Department of Obstetrics & Gynecology, Ioannina University Hospital, Ioannina, Greece

6. Unit for Human Reproduction, 1st Department of Obstetrics & Gynecology, Medical School, Aristotle University of Thessaloniki, Thessaloniki, Greece

7. Laboratory of Forensic Medicine & Toxicology, Medical School, Aristotle University of Thessaloniki, Thessaloniki, Greece

Abstract

Aim: Till now there is very limited knowledge on the molecular content of coelomic fluid and cells. This study presents the first attempt to elucidate the metabolic profile of such samples. Methodology: Samples were collected via coelocentesis from 41 women during the first trimester of gestation. Metabolic content was assessed using four different analytical platforms. For targeted analysis a hydrophilic interaction chromatography ultra high performance LC–MS/MS method was applied. Holistic analysis performed by GC–MS, NMR spectroscopy and ion cyclotron ultra-high resolution MS (FT–ICR–MS) instrumentation. Results & Conclusions: Our observations suggest coelomic fluid and cells as promising biosamples, rich in metabolites with potential use in mammalian system biology studies.

Publisher

Future Science Ltd

Subject

Medical Laboratory Technology,Clinical Biochemistry,General Pharmacology, Toxicology and Pharmaceutics,General Medicine,Analytical Chemistry

Link

http://www.future-science.com/doi/pdf/10.4155/bio-2016-0223

Reference28 articles.

1. Fluid compartments of the embryonic environment

2. Short-term safety of celocentesis for the mother and the fetus

3. Coelocentesis: a new technique for early prenatal diagnosis

4. Quantitative evaluation of fluorescencein situ hybridization (FISH) signals in uncultured coelomic cells

5. Letter to the Editor. Turner's syndrome 45,X found by coelocentesis

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