Uncovering a novel function of the CCR4-NOT complex in phytochrome A-mediated light signalling in plants

Author:

Schwenk Philipp12,Sheerin David J1,Ponnu Jathish3ORCID,Staudt Anne-Marie1,Lesch Klara L124,Lichtenberg Elisabeth1,Medzihradszky Katalin F5,Hoecker Ute3,Klement Eva5,Viczián András6ORCID,Hiltbrunner Andreas17ORCID

Affiliation:

1. Institute of Biology II, Faculty of Biology, University of Freiburg, Freiburg, Germany

2. Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Freiburg, Germany

3. Institute for Plant Sciences and Cluster of Excellence on Plant Sciences (CEPLAS), University of Cologne, Cologne, Germany

4. Internal Medicine IV, Department of Medicine, Medical Center, University of Freiburg, Freiburg, Germany

5. Laboratory of Proteomics Research, Biological Research Centre, Szeged, Hungary

6. Institute of Plant Biology, Biological Research Centre, Szeged, Hungary

7. Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Freiburg, Germany

Abstract

Phytochromes are photoreceptors regulating growth and development in plants. Using the model plant Arabidopsis, we identified a novel signalling pathway downstream of the far-red light-sensing phytochrome, phyA, that depends on the highly conserved CCR4-NOT complex. CCR4-NOT is integral to RNA metabolism in yeast and animals, but its function in plants is largely unknown. NOT9B, an Arabidopsis homologue of human CNOT9, is a component of the CCR4-NOT complex, and acts as negative regulator of phyA-specific light signalling when bound to NOT1, the scaffold protein of the complex. Light-activated phyA interacts with and displaces NOT9B from NOT1, suggesting a potential mechanism for light signalling through CCR4-NOT. ARGONAUTE 1 and proteins involved in splicing associate with NOT9B and we show that NOT9B is required for specific phyA-dependent alternative splicing events. Furthermore, association with nuclear localised ARGONAUTE 1 raises the possibility that NOT9B and CCR4-NOT are involved in phyA-modulated gene expression.

Funder

Deutsche Forschungsgemeinschaft

Hungarian Scientific Research Fund

Economic Development and Innovation Operative Program

Fonds National de la Recherche Luxembourg

Publisher

eLife Sciences Publications, Ltd

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience

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