Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana-Reference-Cited by-同舟云学术

Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana

Published:2003-08 Issue:5633 Volume:301 Page:653-657
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Alonso José M.¹²³,Stepanova Anna N.¹²³,Leisse Thomas J.¹²³,Kim Christopher J.¹²³,Chen Huaming¹²³,Shinn Paul¹²³,Stevenson Denise K.¹²³,Zimmerman Justin¹²³,Barajas Pascual¹²³,Cheuk Rosa¹²³,Gadrinab Carmelita¹²³,Heller Collen¹²³,Jeske Albert¹²³,Koesema Eric¹²³,Meyers Cristina C.¹²³,Parker Holly¹²³,Prednis Lance¹²³,Ansari Yasser¹²³,Choy Nathan¹²³,Deen Hashim¹²³,Geralt Michael¹²³,Hazari Nisha¹²³,Hom Emily¹²³,Karnes Meagan¹²³,Mulholland Celene¹²³,Ndubaku Ral¹²³,Schmidt Ian¹²³,Guzman Plinio¹²³,Aguilar-Henonin Laura¹²³,Schmid Markus¹²³,Weigel Detlef¹²³,Carter David E.¹²³,Marchand Trudy¹²³,Risseeuw Eddy¹²³,Brogden Debra¹²³,Zeko Albana¹²³,Crosby William L.¹²³,Berry Charles C.¹²³,Ecker Joseph R.¹²³

Affiliation:

1. Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.

2. National Research Council Plant Biotechnology Institute, Saskatoon, SK, S7N 0W9 Canada.

3. Department of Family/Preventive Medicine, University of California, San Diego, CA 92093, USA.

Abstract

Over 225,000 independent Agrobacterium transferred DNA (T-DNA) insertion events in the genome of the reference plant Arabidopsis thaliana have been created that represent near saturation of the gene space. The precise locations were determined for more than 88,000 T-DNA insertions, which resulted in the identification of mutations in more than 21,700 of the ∼29,454 predicted Arabidopsis genes. Genome-wide analysis of the distribution of integration events revealed the existence of a large integration site bias at both the chromosome and gene levels. Insertion mutations were identified in genes that are regulated in response to the plant hormone ethylene.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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