Functional Characterization of the S. cerevisiae Genome by Gene Deletion and Parallel Analysis-Reference-Cited by-同舟云学术

Functional Characterization of the S. cerevisiae Genome by Gene Deletion and Parallel Analysis

Published:1999-08-06 Issue:5429 Volume:285 Page:901-906
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Winzeler Elizabeth A.¹,Shoemaker Daniel D.²,Astromoff Anna¹,Liang Hong¹,Anderson Keith¹,Andre Bruno³,Bangham Rhonda⁴,Benito Rocio⁵,Boeke Jef D.⁶,Bussey Howard⁷,Chu Angela M.¹,Connelly Carla⁶,Davis Karen¹,Dietrich Fred⁸,Dow Sally Whelen²,El Bakkoury Mohamed⁹,Foury Françoise¹⁰,Friend Stephen H.²,Gentalen Erik¹¹,Giaever Guri¹,Hegemann Johannes H.¹²,Jones Ted¹,Laub Michael¹,Liao Hong⁴,Liebundguth Nicole⁸,Lockhart David J.¹¹,Lucau-Danila Anca¹⁰,Lussier Marc⁷,M'Rabet Nasiha³,Menard Patrice⁷,Mittmann Michael¹¹,Pai Chai¹,Rebischung Corinne⁸,Revuelta Jose L.⁵,Riles Linda¹³,Roberts Christopher J.²,Ross-MacDonald Petra⁴,Scherens Bart⁹,Snyder Michael⁴,Sookhai-Mahadeo Sharon⁶,Storms Reginald K.⁷,Véronneau Steeve⁷,Voet Marleen¹⁴,Volckaert Guido¹⁴,Ward Teresa R.²,Wysocki Robert¹⁰,Yen Grace S.¹,Yu Kexin⁶,Zimmermann Katja¹²,Philippsen Peter⁸,Johnston Mark¹³,Davis Ronald W.¹

Affiliation:

1. Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305–5307, USA.

2. Rosetta Inpharmatics Inc., 12040 115th Street NE, Kirkland, WA 98034, USA.

3. Universite Libre de Bruxelles, Laboratoire de Physiologie Cellulaire et de Genetique des Levures, Campus Plaine, Brussels CP244, Belgium.

4. Department of Molecular, Cellular & Developmental Biology, Yale University, New Haven, CT 06520–8103, USA.

5. Dipartimento de Microbiologia y Genetica, Universidad de Salamanca, Edificio Depatamental 323/CSIC, Campus Miguel de Unamuno, E-37007 Salamanca, Spain.

6. Department of Molecular Biology & Genetics, Johns Hopkins University School of Medicine, 617 Hunterian Building, 725 North Wolfe Street, Baltimore, MD 21205–2185, USA.

7. Department of Biology, McGill University, Montreal, PQ, Canada H3A 1B1.

8. Biozentrum, Department of Molecular Microbiology, Biozentrum, University of Basel, Switzerland.

9. IRMW-ULB, Avenue E. Gryson, 1, B-1070 Brussels, Belgium.

10. FYSA-UCL, Place Croix du Sud, 2/20, 1348-Louvain-la-Neuve, Belgium.

11. Affymetrix, 3380 Central Expressway, Santa Clara, CA 95051, USA.

12. Institut für Mikrobiologie, Geb. 26.12.01 Raum 64, Universitaetsstrasse 1, D-40225 Düsseldorf, Germany.

13. Department of Genetics, Washington University Medical School, St. Louis, MO 63110, USA.

14. Katholieke Universiteit Leuven, Laboratory of Gene Technology, Kardinaal Mercierlaan 92, B-3001 Leuven, Belgium.

Abstract

The functions of many open reading frames (ORFs) identified in genome-sequencing projects are unknown. New, whole-genome approaches are required to systematically determine their function. A total of 6925 Saccharomyces cerevisiae strains were constructed, by a high-throughput strategy, each with a precise deletion of one of 2026 ORFs (more than one-third of the ORFs in the genome). Of the deleted ORFs, 17 percent were essential for viability in rich medium. The phenotypes of more than 500 deletion strains were assayed in parallel. Of the deletion strains, 40 percent showed quantitative growth defects in either rich or minimal medium.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference48 articles.

1. Genome cross-referencing and XREFdb: Implications for the identification and analysis of genes mutated in human disease

2. Human genetic diseases: a cross-talk between man and yeast

3. Genetic footprinting: a genomic strategy for determining a gene's function given its sequence.

4. Large-scale analysis of gene expression, protein localization, and gene disruption in Saccharomyces cerevisiae.

5. A multipurpose transposon system for analyzing protein production, localization, and function in Saccharomyces cerevisiae

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