Enhanced single RNA imaging reveals dynamic gene expression in live animals-Reference-Cited by-同舟云学术

Enhanced single RNA imaging reveals dynamic gene expression in live animals

Published:2023-03-03 Issue: Volume:12 Page:
ISSN:2050-084X
Container-title:eLife
language:en
Short-container-title:

Author:

Hu Yucen¹,Xu Jingxiu²,Gao Erqing²,Fan Xueyuan¹^ORCID,Wei Jieli¹,Ye Bingcheng¹,Xu Suhong²³^ORCID,Ma Weirui¹^ORCID

Affiliation:

1. Zhejiang Provincial Key Laboratory of Cancer Molecular Cell Biology, Life Sciences Institute, Zhejiang University

2. International Biomedicine-X research center of the Second Affiliated Hospital, Zhejiang University

3. Center for Stem Cell and Regenerative Medicine and Department of Burn and wound repair of the Second Affiliated Hospital, Zhejiang University School of Medicine

Abstract

Imaging endogenous mRNAs in live animals is technically challenging. Here, we describe an MS2-based signal amplification with the Suntag system that enables live-cell RNA imaging of high temporal resolution and with 8xMS2 stem-loops, which overcomes the obstacle of inserting a 1300 nt 24xMS2 into the genome for the imaging of endogenous mRNAs. Using this tool, we were able to image the activation of gene expression and the dynamics of endogenous mRNAs in the epidermis of live C. elegans.

Funder

National Natural Science Foundation of China

Leading innovation and entrepreneurship

Zhejiang University

National Key R&D Program of China

Zhejiang Province Natural Science Foundation

Publisher

eLife Sciences Publications, Ltd

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience

Link

https://cdn.elifesciences.org/articles/82178/elife-82178-v3.pdf