Longitudinal map of transcriptome changes in the Lyme pathogen Borrelia burgdorferi during tick-borne transmission

Author:

Sapiro Anne L1ORCID,Hayes Beth M1ORCID,Volk Regan F2ORCID,Zhang Jenny Y1ORCID,Brooks Diane M3,Martyn Calla1,Radkov Atanas1,Zhao Ziyi1,Kinnersley Margie3,Secor Patrick R3ORCID,Zaro Balyn W2ORCID,Chou Seemay1

Affiliation:

1. Department of Biochemistry & Biophysics, University of California, San Francisco

2. Department of Pharmaceutical Chemistry and Cardiovascular Research Institute, University of California, San Francisco

3. Division of Biological Sciences, University of Montana

Abstract

Borrelia burgdorferi (Bb), the causative agent of Lyme disease, adapts to vastly different environments as it cycles between tick vector and vertebrate host. During a tick bloodmeal, Bb alters its gene expression to prepare for vertebrate infection; however, the full range of transcriptional changes that occur over several days inside of the tick are technically challenging to capture. We developed an experimental approach to enrich Bb cells to longitudinally define their global transcriptomic landscape inside nymphal Ixodes scapularis ticks during a transmitting bloodmeal. We identified 192 Bb genes that substantially change expression over the course of the bloodmeal from 1 to 4 days after host attachment. The majority of upregulated genes encode proteins found at the cell envelope or proteins of unknown function, including 45 outer surface lipoproteins embedded in the unusual protein-rich coat of Bb. As these proteins may facilitate Bb interactions with the host, we utilized mass spectrometry to identify candidate tick proteins that physically associate with Bb. The Bb enrichment methodology along with the ex vivo Bb transcriptomes and candidate tick interacting proteins presented here provide a resource to facilitate investigations into key determinants of Bb priming and transmission during the tick stage of its unique transmission cycle.

Funder

Life Sciences Research Foundation

Arnold and Mabel Beckman Foundation

Montana INBRE

Chan Zuckerberg Initiative

Pew Charitable Trusts

Publisher

eLife Sciences Publications, Ltd

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine,General Neuroscience

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