Bioanalytical Method Development and Validation of UV Spectrophotometric Method for Estimation of Metformin Hydrochloride in Urine Sample-Reference-Cited by-同舟云学术

Bioanalytical Method Development and Validation of UV Spectrophotometric Method for Estimation of Metformin Hydrochloride in Urine Sample

Published:2024-04-30 Issue: Volume: Page:93-96
ISSN:0974-4150
Container-title:Asian Journal of Research in Chemistry
language:en
Short-container-title:AJRC

Author:

Shinde G. S.¹,Jadhav R. S.¹,Kote R. B.¹,Kadam A. J.²,Bankar A. A.²

Affiliation:

1. Department of Pharmaceutics, Institute of Pharmacy Loni. Tal-Rahata, District - Ahmednagar. (M.S.) 413736.

2. Department of Pharmaceutical Chemistry, Institute of Pharmacy Loni, Taluka-Rahata District-Ahmednagar. (M.S.) 413736.

Abstract

Purpose: To develop a simple, sensitive, accurate, precise and rapid UV spectrophotometric method for the determination of metformin in human urine. Methods: Extraction of metformin from urine samples was done by Dilute and shoot method in which urine was diluted up to 1:10 ratio to avoid interference of matrix. For estimation of Metformin hydrochloride, the solvent system employed was water. The metformin hydrochloride was scanned in range of 200nm to 400 nm and wavelength of detection (λdet) was found to be 234 nm. Result: The calibration curve was linear in the range of 1-17 μg/ml. the recovery and assay studies of metformin hydrochloride were within 93-94.85% indicating that the proposed method can be estimation of metformin hydrochloride

Publisher

A and V Publications

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