Platelet Derived Growth Factor Expression after Administration of Okra Fruit Extract on Diabetic Wistar rats

Abstract

Background: Increased AGEs in diabetics promote a protracted inflammatory response and macrophage malfunction in growth factor release, one of which being Platelet Derived Growth Factor (PDGF). The disruption of proliferation and angiogenesis is influenced by a reduction in PDGF expression. Okra (Abelmoschus esculentus) fruit extract possesses antioxidant, anti-inflammatory, and anti-diabetic properties. This extract aids in the inflammatory process, macrophage proliferation, and immunomodulatory macrophage roles in the production of growth factors. Purpose: The goal of this study was to observed if giving an okra fruit extract to a diabetic Wistar rat will improve the expression of PDGF in the wound healing process following tooth extraction. Methods: 24 Wistar rats were divided into 2 groups: Control Group (Streptozotocin-induced), and Treatment Group (Streptozotocin-induced and treated with okra fruit extract 250mg/kgB Woral sonde once a day after extraction). Extraction was performed on the lower jaw left incisor. Four rats from each group were sacrificed on day 3, 5, and 7 after the extraction. To evaluate the number of PDGF expression, the socket on the left lower jaw was cut open and immunohistochemistry was performed. Results: There was a significant difference between the number of PDGF expressions on the treatment group on day 3 (10.92245±0.80934), day 5 (15.71875±1.31646) day 7(17.71875±1.24506) with control group on day 3(3.71875±1.65004), 5(7.94791± 0.51637), and 7(7.89062±0.61740). Conclusion: On diabetic Wistar rats, okra fruit extract modulates the increase in PDGF expression in wounds after tooth extraction.