Comparative Study of The Chemical Composition and Pharmacological Activity of Three Chemotypes of Peppermint

Abstract

The aim of the work was to study the composition of the alcohol extract, antioxidant activity and pharmacological properties of peppermint leaves of three forms - menthol, linalool and carvone. Materials and methods: Chromatography-mass-spectrometric analysis was performed on an Agilent Technologies device. For the model evaluation of antioxidant activity, the aerometric method for measuring the total antioxidant activity was used, implemented in the TsvetYauza-01-AA device. Tonic activity was studied in terms of the duration of swimming of laboratory animal (male mice) with a load of 7% of the animal's body weight until complete fatigue - "The mouse forced swim test". The discussion of the results. When studying the chemical composition of medicinal plant raw materials of peppermint leaves of menthol, linalool, carvone types, more than 50 compounds were identified. A comparative analysis showed that the content of menthol in peppermint leaves of the menthol type can exceed the content of menthol in other chemotypes by ten or more times. At the same time, the tonic effect of an aqueous extract from peppermint leaves is mostly associated with the menthol. It should be taken into account when using this type of raw material in the manufacturing. Findings. The raw material of peppermint leaves may have a different degree of pharmacological activity depending on the chemotype, which should be taken into consideration when using this type of raw material.