Qualitative Phytochemical Analysis in Determination of Antioxidant Activity of Methanolic Extract of Oenothera biennis by GCMS – A Preliminary Research Study

Abstract

Introduction and Aim: Oenothera biennis an important medicinal plant which belongs to Onagraceae family. It is used for various medicinal purposes in ayurvedic medicine and herbal remedy. The aim of the present study was to evaluate the antioxidant activity of phenol and flavonoid extract of plant of O. biennis and GC-MS analysis for active compound identification. Materials and Methods: Radical scavenging assay and reduction assay methods were used for antioxidant activity. The antioxidant capacity of methanolic plant extractHP-5 column was used for GCMS analysis. Results: The IC50 of DPPH radical scavenging activity of methanol leaves extract of O. biennis was 31.43µg/mL concentration, IC50value of superoxide radical scavenging activity was 37.71µg/mL concentration. The RC50 of phosphomolybdenum reduction of methanolic plant extract of O. biennis was 49.90µg/mL concentration and the RC50of Fe3+ reduction was 37.25µg/mL concentrations. Antioxidant compounds such as Phenol, 2, 6-bis(1,1-dimethyl)-4-[(4-hydroxy-3,5-dimethylphenyl)methyl]-, Cromaril and Oleic acid were eluted by GCMS analysis. Conclusion: The data showed that the methanolic plant extract of O. biennis has significant antioxidant activity. The flavone compounds identified in GCMS could be responsible for antioxidant activity. Further research work needed to isolate active compounds to kill diseases.