Affiliation:
1. Research Scholar, Department of Postgraduate Studies and Research in Biosciences, Mangalore University, Mangalagangothri - 574199, Karnataka, India.
2. Professor, Department of Postgraduate Studies and Research in Biosciences, Mangalore University, Mangalagangothri - 574199, Karnataka, India.
Abstract
Majority of the modern medicines are derived from bioactive constituents of medicinal plants. The current investigation was designed to evaluate the phytochemical constituents and antioxidant activity of methanolic extract of leaves of Ricinus communis L. Qualitative analysis of phytochemicals viz. tannins, phlobatannins, saponins, flavonoids, steroids, alkaloids, coumarin, terpenoids and cardiac glycosides and quantitative analysis of total phenolics, alkaloids, tannins and flavonoids were performed by using standard protocol. Antioxidant activity was studied through DPPH, ABTS, phosphomolybdenum and reducing power assay. To confirm the phytoconstituents of the leaf extracts, Gas chromatography-Mass spectrometry (GC-MS) method was followed. In the present study, qualitative and quantitative analysis revealed the presence of major class of phytochemicals. Quantitative analysis of alkaloids, flavonoids, tannins and phenols were 32.28 ± 0.28 mg/g, 256.29 ± 0.21 mg QE/g, 175.59 ± 0.78 mg TAE/g, 273.35 ± 2.33 mg GAE/g respectively. The extract showed a good scavenging activity with DPPH (84.22 ± 0.81 %) and the GC-MS chromatogram revealed the presence of 23 peaks with different compounds and Ricinine was found to be the major compound. The presence of these bioactive molecules in R. communis may provide the scientific evidences for the medicinal value and other biological properties.
Subject
Pharmacology (medical),Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
Reference42 articles.
1. Afroz Patan M. Saranya S. Vignesh A. Bharathi G. Vikram P. Yuvaraj M. Vijeyaanandhi. Qualitative Phytochemical analysis in determination of antioxidant activity of methanolic extract of Oenothera biennis by GCMS- a preliminary research study. Research Journal of Pharmacy and Technology. 2021; 14(7): 3744-0. Doi.org/10.52711/0974-360X.2021.00648
2. Vasu K. Goud JV. Suryam A. Singara. Chary MA. Biomolecular and phytochemical analysis of three aquatic angiosperm. African journal of Microbiology Research. 2009; 3(8): 418-421. Doi.org/10.5897/AJMR.9000134
3. Matthew OO. Olusola L. Matthew OA. Preliminary study of hypoglycaemic and hypolipidemic activity of aqueous root extract of Ricinus communis in alloxan-induced diabetic rats. Journal of physiology and pharmacology advances. 2012; 2(10): 354-359.
4. Ravishankar K. Indira K. Vijay Bhaskar R. In vivo hepatoprotective activity of Ricinus communis Linn leaf extract against CCl4 induced hepatic damage in albino rats. International Journal of Bio Pharma Research. 2012; 3(3): 444-449.
5. Hussein HM. Hameed IH. Ubaid JM. Analysis of the secondary metabolite products of Ammi majus and evaluation anti-insect activity. International journal of pharmacognosy and phytochemical research. 2016; 8(8): 1192-1189.