Phytochemical and Antibacterial Analysis of the Formulated Cream of Black Cumin Honey

Abstract

This study reports the bioactive compound of black cumin honey and investigates the antibacterial activity against Propionibacterium acnes and Pseudomonas aeruginosa. HPLC and GC-MS methods were used to analyze the bioactive compounds of the black cumin honey. The formulated cream of black cumin honey was prepared in an oil-in-water type cream with various concentrations of 1%, 5%, 10%, 15%, 20%, and 30% (w/w). Antibacterial effects against Propionibacterium acnes and Pseudomonas aeruginosa were examined by the disc diffusion method. Physical stability of formulated cream included organoleptic, homogeneity, and pH measurement test. The result of GC-MS analysis of black cumin honey showed 25 compounds: organic fatty acids, saccharides, and amino acid. HPLC analysis of flavonoid content showed quercetin as the bioactive compound at a concentration of 0.223 mg/g. Disc diffusion analysis showed great inhibitory potential against P. acnes and P. aeruginosa in 30% concentration with d = 11.2 mm and 10.8 mm, respectively. It can be concluded that the formulated cream of black cumin honey showed a great potential application as antibacterial agents of P. acnes and P. aeruginosa.