Antibacterial activity of Chito-oligosaccharides derived from Fish Scales

Abstract

This study aimed to evaluate the antibacterial activity of chito-oligosaccharides (COS) from Tilapia fish scales. There is a massive potential in sustainably prospecting from these resources: algae, vertebrate, crustaceans, molluscs, residues from fish farms, and fisheries such as bones, skin, and fins, internal organs and non-bio-degradable substances such as scales—the production COS achieved by chemical hydrolysis involving demineralization, deproteinization and deacetylation. The antibacterial activities performed against Staphylococcus aureus, Bacillus cereus, Streptococcus agalactiea, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi. The bacteriostatic and bactericidal effect measured by taking Minimum Inhibitory Concentrations (MIC), and the Minimum Bactericidal Concentrations (MBC). Chitin yielded 32.74% from 100 grams of dried fish scales in this study, whereas Chito-oligosaccharides yielded 24.12% from 100 grams of chitin. The ratio of MBC/MIC determination reveals the bacteriostatic and bactericidal effect of a COS. The bactericidal properties were visible in COS. The potential antibacterial effect of COS obtained by Tilapia fish scales could be utilized for biomedical purposes such as wound healing.