Biological activity of Green Nanoparticle Synthesized from Glycyrrhiza glabra in vitro and in vivo

Abstract

Background and objective: Glycyrrhiza glabra is one of the useful medicinal plants with increasing demand for (herbal medicines, health products, pharmaceuticals). Glycyrrhiza glabra used in traditional medicine across the world for its ethnopharmacological value. Materials and Method: this study was included the evaluation of the effects of green synthetic nanoparticles of Glycyrrhiza glabra aqueous extract loaded with silver nitrate on antibacterial activity and anti-inflammatory activity through burn healing effect. The antibacterial activity of green synthetic silver nanoparticles was studied against one type of Gram-negative (E.coli) and Gram-positive (staphylococcus aureus). Also, the effects of burns healing effect of green synthetic silver nanoparticle was evaluated using albino male mice. The activity of green synthetic nanoparticle at (1.5mM) was investigated in compared with sliver sulfadiazine as positive control and negative control mice (without any treatment) by determining days require for healing. Results: the result showed that different nanoparticles concentrations (1, 1.5, 1.75, 2 mM) can inhibit the bacterial isolate with varying zones of (17, 20, 12,17mm) for Gram-positive Staphylococcus aureus) and (10, 22, 10, 22mm) for Gram-negative (E.coli) at (1, 1.5, 1.75, 2mM) respectively. Also, the results showed that green synthetic nanoparticle could heal burns in 12 day compared to 14 days for sliver sulfadiazine and 18 days for negative control. Conclusion: green synthetic nanoparticle possessed antibacterial and anti-inflammatory activity due to active constituents of plant.