Selection and validation of endogenous reference genes for RT-qPCR normalization in different stresses and tissues of the tiger milk mushroom, Pleurotus tuber-regium
Author:
Affiliation:
1. College of Food Science and Technology, Huazhong Agricultural University
2. Key Laboratory of Agro-Microbial Resources and Utilization, Ministry of Agriculture
Publisher
The Mycological Society of Japan
Subject
Ecology, Evolution, Behavior and Systematics
Link
https://www.jstage.jst.go.jp/article/mycosci/62/5/62_MYC542/_pdf
Reference27 articles.
1. Andersen, C. L., Jensen, J. L., & Ørntoft, T. F. (2004). Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Research, 64, 5245–5250. https://doi.org/10.1158/0008-5472.CAN-04-0496
2. Bamigboye, C. O., Oloke, J. K., & Dames, J. F. (2019). Development of high yielding strain of Pleurotus tuber-regium: fructification, nutritional and phylogenetic studies. Journal of Food Science and Technology, 56, 3597–3608. https://doi.org/10.1007/s13197-019-03786-7
3. Castanera, R., López-Varas, L., Pisabarro, A. G., & Ramírez, L. (2015). Validation of reference genes for transcriptional analyses in Pleurotus ostreatus by using reverse transcription-quantitative PCR. Applied and Environmental and Microbiology, 81, 4120–4129. https://doi.org/10.1128/AEM.00402-15
4. de Oliveira, L. A., Breton, M. C., Bastolla, F. M., Camargo, S. D. S., Margis, R., Frazzon, J., & Pasquali, G. (2012). Reference genes for the normalization of gene expression in Eucalyptus species. Plant and Cell Physiology, 53, 405–422. https://doi.org/10.1093/pcp/pcr187
5. Derveaux, S., Vandesompele, J., & Hellemans, J. (2010). How to do successful gene expression analysis using real-time PCR. Methods, 50, 227–230. https://doi.org/10.1016/j.ymeth.2009.11.001
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1. Development of reference genes for RT-qPCR analysis of gene expression in Pleurotus pulmonarius for biotechnological applications;Scientific Reports;2023-07-29
2. Relationship between antioxidant enzymes and sclerotial formation of Pleurotus tuber-regium under abiotic stress;Applied Microbiology and Biotechnology;2023-01-14
3. Genome and Comparative Transcriptome Dissection Provide Insights Into Molecular Mechanisms of Sclerotium Formation in Culinary-Medicinal Mushroom Pleurotus tuber-regium;Frontiers in Microbiology;2022-02-17
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