Investigation of the inhibitory activity of extracts, fractions and secondary metabolites of Silene spp. (Caryophylaceae) and Serratula cupuliformis (Asteraceae) on the «entry» of herpes simplex type 2 into sensitive cells of the Vero line-Reference-Cited by-同舟云学术

Investigation of the inhibitory activity of extracts, fractions and secondary metabolites of Silene spp. (Caryophylaceae) and Serratula cupuliformis (Asteraceae) on the «entry» of herpes simplex type 2 into sensitive cells of the Vero line

Published:2024-04-07 Issue:1 Volume:19 Page:30-46
ISSN:2413-0958
Container-title:South of Russia: ecology, development
language:
Short-container-title:Ûg Ross.: èkol. razvit.

Author:

Каzachinskaia E. I.¹^ORCID,Zibareva L. N.²^ORCID,Filonenko E. S.²^ORCID,Ivanova A. V.³^ORCID,Коnonova Yu. V.⁴^ORCID,Chepurnov А. A.⁴^ORCID,Shestopalov A. M.⁴^ORCID

Affiliation:

1. Research Institute of Virology, Federal Research Center of Fundamental and Translational Medicine; Vector State Research Centre of Virology and Biotechnology, Federal Service for the Oversight of Consumer Protection and Welfare (Rospotrebnadzor)

2. National Research Tomsk State University

3. Vector State Research Centre of Virology and Biotechnology, Federal Service for the Oversight of Consumer Protection and Welfare (Rospotrebnadzor)

4. Research Institute of Virology, Federal Research Center of Fundamental and Translational Medicine

Abstract

In vitro analysis of the inhibitory activity of extracts, fractions and secondary metabolites of plants of the genus Silene (Caryophylaceae) and Serratula cupuliformis (Asteraceae) to the «entry» of herpes simplex type 2 (HSV‐2) into sensitive cells of the Vero line.Ethanol extracts and butanol fractions of Silene spp. and Serratula cupuliformis were prepared. The flavonoid shaftoside and the ecdysteroid 20‐hydroxyecdysone from Lychnis chalcedonica were isolated. For analysis of biologically active substances (BAS) the HPLC method was used. The samples were dissolved in distilled water or DMSO. The laboratory strain MS HSV‐2 was grown on Vero cell culture. In vitro analysis of the inhibitory activity of the herbal preparations on «entry» of HSV‐2 was performed in Vero cell culture by direct inactivation (neutralisation) of virions with 103 PFU /ml.Effective concentrations (EC50) have been identified in the range of 50 % from 2.12±0.47 to 180.99±49.24 μg/ml when preparations were dissolved in water and from 1.99±0.44 to 57.42±14.74 μg/ml when they were dissolved in DMSO. Such results for some samples is comparable to the EC50 of comparison preparations (dry ethanol extracts of spice of cloves, chaga and licorice root). The results obtained suggest the presence of BAS in the herbal preparations studied that act destructively on HSV‐2 virions and affect one of the main stages of its «life» cycle – the «entry» of the virus into sensitive cells.

Publisher

Institute of Applied Ecology

Reference57 articles.

1. Sait Mezhdunarodnogo komiteta po taksonomii virusov (ICTV, 2021) [Website of the International Committee on Virus Taxonomy]. Available at: https: ictv.global/taxonomy (accessed 01.12.2023)

2. Gatherer D., Depledge D.P., Hartley C.A., Szpara M.L., Vaz P.K., Benkő M., Brandt C.R., Bryant N.A., Dastjerdi A., Doszpoly A., Gompels U.A., Inoue N., Jarosinski K.W., Kaul R., Lacoste V., Norberg P., Origgi F.C., Orton R.J., Pellett P.E., Schmid D.S., Spatz S.J., Stewart J.P., Trimpert J., Waltzek T.B., Davison A.J. ICTV Virus Taxonomy Profile: Herpesviridae 2021. Journal of General Virology, 2021, vol. 102, no. 10, article id: 001673. DOI: 10.1099/jgv.0.001673

3. Connolly S.A., Jardetzky T.S., Longnecker R. The structural basis of herpesvirus entry. Nature Reviews Microbiology, 2021, vol. 19, no. 2, pp. 110–121. DOI: 10.1038/s41579‐02000448‐w

4. Jambunathan N., Clark C.M., Musarrat F., Chouljenko V.N., Rudd J., Kousoulas K.G. Two Sides to Every Story: Herpes Simplex Type‐1 Viral Glycoproteins gB, gD, gH/gL, gK, and Cellular Receptors Function as Key Players in Membrane Fusion. Viruses, 2021, vol. 13, no. 9, article id: 1849. DOI: 10.3390/v13091849

5. de Souza Carneiro V.C., Pereira J.G., de Paula V.S. Family Herpesviridae and neuroinfections: current status and research in progress. Memórias do Instituto Oswaldo Cruz, 2022, vol. 117, article id: e220200. DOI: 10.1590/007402760220200