Investigation of the inhibitory activity of extracts, fractions and secondary metabolites of Silene spp. (Caryophyllaceae) and Serratula cupuliformis (Asteraceae) on the replication of SARS-CoV-2 coronavirus-Reference-Cited by-同舟云学术

Investigation of the inhibitory activity of extracts, fractions and secondary metabolites of Silene spp. (Caryophyllaceae) and Serratula cupuliformis (Asteraceae) on the replication of SARS-CoV-2 coronavirus

Published:2023-03-30 Issue:1 Volume:18 Page:62-81
ISSN:2413-0958
Container-title:South of Russia: ecology, development
language:
Short-container-title:Ûg Ross.: èkol. razvit.

Author:

Kazachinskaia E. I.¹^ORCID,Zibareva L. N.²^ORCID,Filonenko E. S.²^ORCID,Ivanova A. V.³^ORCID,Gadzhieva M. M.⁴^ORCID,Bekshokov K. K.⁵^ORCID,Kononova Yu. V.⁶^ORCID,Chepurnov A. A.⁷^ORCID,Shestopalov A. М.⁷^ORCID

Affiliation:

1. Research Institute of Virology, Federal Research Centre of Fundamental and Translational Medicine, Siberian Branch, Russian Academy of Sciences; Vector State Research Centre of Virology and Biotechnology

2. National Research Tomsk State University

3. Vector State Research Centre of Virology and Biotechnology

4. N.I. Prigorov Russian National Research Medicine University

5. I.M. Sechenov First Moscow State Medical University, Russian Ministry of Health

6. Research Institute of Virology, Federal Research Centre of Fundamental and Translational Medicine, Siberian Branch, Russian Academy of Sciences

7. Research Institute of Virology, Federal Research Centre of Fundamental and Translational Medicine, Siberian Branch, Russian Academy of Sciences; Dagestan State University

Abstract

Aim. In vitro analysis of the inhibitory activity of extracts, fractions and secondary metabolites of plants of the genus Silene [Caryophylaceae] and Serratula cupuliformis [Asteraceae) on the replication of SARS-CoV-2.Material and Methods. Silene spp. and Serratula cupuliformis of the Siberian Botanical Garden of National Research Tomsk State University were used. Ethanol extracts and butanol fractions of Silene spp. were prepared. The flavonoid shaftoside and the ecdysteroid 20-hydroxyecdysone from Lychnis chalcedonica were isolated. Analysis of BAS was carried out by the HPLC method. In vitro analysis of the inhibitory activity of extracts on SARS-CoV-2 replication was performed in Vero cell culture by direct inactivation [neutralization) of virions. Comparison samples were dry ethanol extracts of chaga [Inonotus obliquus, Basidiomycota), spices of cloves [Syzygium aromaticum, Myrtaceae) and root of licorice [Glycyrrhiza glabra L., Fabaceae).Results. The inhibitory activity of ethanol extracts and butanol fractions of Silene spp., as well as individual compounds [shaftozide and 20-E) was revealed in the range of 50% effective concentrations [EC50) when dissolved in water from 339.85±83.92 pg/ml to 1.59±0.39 pg/ml and when dissolved in DMSO from 119.34±26.34 pg/ml to 2.22±0.57 pg/ml, respectively. The butanol fraction of Serratula cupuliformis was active with EC50=21.74±4.80 and 27.42±6.05 pg/mL. These results for some samples of Silene spp. and Serratula cupuliformis are comparable to the EC50 values of the comparators.Conclusion. The results obtained suggest the presence of biologically active substances in the herbal preparations studied that act destructively on virions of SARS-CoV-2 and affect one of the main stages of its "life" cycle - on the attachment to receptors of sensitive cells.

Publisher

Institute of Applied Ecology

Subject

Ecology,Geography, Planning and Development,Ecology, Evolution, Behavior and Systematics

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1. Investigation of the inhibitory activity of extracts, fractions and secondary metabolites of Silene spp. (Caryophylaceae) and Serratula cupuliformis (Asteraceae) on the «entry» of herpes simplex type 2 into sensitive cells of the Vero line;South of Russia: ecology, development;2024-04-07