Tryptophan-Restriction Diets Help to Maintain L-Tryptophan Homeostasis in Tryptophan 2,3-Dioxygenase Knockout Mice

Author:

Maeta Akihiro1,Fukuwatari Tsutomu1,Funakoshi Hiroshi2,Nakamura Toshikazu3,Shibata Katsumi1

Affiliation:

1. Department of Nutrition, School of Human Cultures, the University of Shiga Prefecture, Hikone, Shiga, Japan.

2. Division of Molecular Regenerative Medicine, Department of Biochemistry and Molecular Biology, Asahikawa, Hokkaido, Japan.

3. Kringle Pharma Joint Research Division for Regenerative Drug Discovery, Center for Advanced Science and Innovation, Osaka University, Osaka, Japan.

Abstract

We hypothesized that the requirements of essential nutrients are dependent upon catabolic abilities. Mice lacking L-tryptophan 2,3-dioxygenase (TDO) are available. The body concentration of L-tryptophan (L-Trp) has been reported to be higher in TDO-deficient mice than in wild-type (WT) mice. We examined the requirement of an appropriate L-Trp level for TDO-deficient mice using several biomarkers. TDO-deficient mice were fed a 10% amino-acid mixture diet containing 0.06%, 0.08%, and 0.17% L-Trp. WT mice fed a 0.17% Trp diet (standard diet) were used as control mice. The concentrations of L-Trp and its metabolites via serotonin were higher in TDO-deficient mice fed the 0.17% L-Trp diet than in WT mice fed the standard diet, but the concentrations were almost identical between TDO-deficient mice fed the 0.06% L-Trp diet and WT mice fed the standard diet. Therefore, as hypothesized, requirements of essential nutrients are dependent on catabolic abilities.

Publisher

SAGE Publications

Subject

Molecular Biology,Biochemistry

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