A Comparison of Hydraulic and Laser Capture Microdissection Methods for Collection of Single B Cells, PCR, and Sequencing of Antibody VDJ

Author:

Obiakor Harold,Sehgal Devinder,Dasso Joseph F.,Bonner Robert F.,Malekafzali Arash,Mage Rose G.

Publisher

Elsevier BV

Subject

Cell Biology,Molecular Biology,Biochemistry,Biophysics

Reference13 articles.

1. Analyses of single B cells by polymerase chain reaction reveal rearranged VH with germline sequences in spleens of immunized adult rabbits: Implications for B cell repertoire maintenance and renewal;Sehgal;J. Immunol.,1998

2. Gene conversion and hypermutation during diversification of VH sequences in developing splenic germinal centers of immunized rabbits;Schiaffella;J. Immunol.,1999

3. Generation of heterogeneous rabbit anti-DNP antibodies by gene conversion and hypermutation of rearranged VL and VH genes during clonal expansion of B-cells in splenic germinal centers;Sehgal;Eur. J. Immunol.,2000

4. Rabbit IgH sequences in appendix germinal centers: VH diversification by gene conversion-like and hypermutation mechanisms;Weinstein;Immunity,1994

5. In situ studies of the primary immune response to (4-hydroxy-3-nitrophenyl)acetyl. III. The kinetics of V region mutation and selection in germinal center B cells;Jacob;J. Exp. Med.,1993

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