Essentials of LC Troubleshooting, Part II: Misbehaving Retention Times

Abstract

Several liquid chromatography (LC) troubleshooting topics never get old because there are some problems that persist in the practice of LC, even as instrument technology improves over time. There are many ways for things to go wrong in an LC system that ultimately manifest as deviations from expected retention times. Developing a short list of the likely causes of these deviations can help streamline our troubleshooting experience when retention-related problems occur.