Affiliation:
1. Vrije Universiteit Brussel
2. Gustavus Adolphus College
Abstract
Choosing a liquid chromatography (LC) column for a particular application can be a surprisingly challenging task. On the one hand, column manufacturers give us many options to choose from, including particle types, pore sizes, particle sizes, and different lengths and diameters. On the other hand, we usually do not have time to experimentally evaluate many combinations of these parameters, and sometimes we end up picking something similar to the columns that are already in the drawer. The “kinetic plot” is a powerful graphical tool that can help leverage the best available theory to help us understand how different combinations of parameters (such as particle size and length) will perform in relation to the time needed to get to a particular column efficiency (and thus resolution), and therefore make well-informed decisions when choosing columns.
Publisher
Multimedia Pharma Sciences, LLC
Reference12 articles.
1. K. Broeckhoven and G. Desmet, Anal. Chem. 93, 257–272 (2021). https://doi.org/10.1021/acs.analchem.0c04466.
2. K. Broeckhoven, S. Eeltink, W. De Malsche, F. Matheuse, G. Desmet, and D. Cabooter, LCGC N. Am. 36, 9–17 (2018).
3. J.J. van Deemter, F.J. Zuiderweg, and A. Klinkenberg, Chem. Eng. Sci. 5, 271–289 (1956). https://doi.org/10.1016/0009-2509(56)80003-1.
4. A. Andrés, K. Broeckhoven, and G. Desmet, Analytica Chimica Acta. 894, 20–34 (2015). https://doi.org/10.1016/j.aca.2015.08.030.
5. G. Desmet, D. Clicq, and P. Gzil, Anal. Chem. 77, 4058–4070 (2005). https://doi.org/10.1021/ac050160z.
Cited by
5 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献