Abstract
This article describes a liquid chromatography–tandem mass spectrometry (LC–MS/MS) method for the analysis of coumarin in various tobacco matrices and electronic cigarette (E-cig) liquids, and highlights the importance of evaluating different MS/MS transitions of an analyte in complex sample matrices to overcome matrix effects. Matrix interfering components were separated from analyte using a C18 ultrahigh-pressure liquid chromatography (UHPLC) column with a larger inner diameter (3.0 mm, or 4.6 mm). Matrix suppressions on analyte responses were corrected by isotope dilution. Four different MS/MS transitions of coumarin were studied in each sample matrix to select a suitable MS/MS transition for analyte quantification based on matrix effects on each MS/MS transition. The method was validated using different tobacco matrices and E-cig liquids.
Publisher
Multimedia Pharma Sciences, LLC
Reference23 articles.
1. Christakopoulos, A.; Feldhusen, K.; Norin, H.; Palmqvist, A.; Wahlberg, I. Determination of Natural Levels of Coumarin in Different Types of Tobacco Using a Mass Fragmentographic Method. J. Agric. Food Chem. 1992, 40 (8), 1358–1361. DOI: 10.1021/jf00020a014
2. Lake, B. G. Coumarin Metabolism, Toxicity, and Carcinogenicity: Relevance for Human Risk Assessment. Food Chem. Toxicol. 1999, 37 (4), 423–453. DOI: 10.1016/s0278-6915(99)00010-1
3. European Council, Council Directive (EEC) No. 88/388 on the Approximation of the Laws of the Member States Relating to Flavourings for Use in Foodstuffs and to Source Materials for Their Production. Off. J. Europ. Comm. 1988, L184, 61–66.
4. U.S. Food and Drug Administration (FDA), Title 21-Food and Drugs, Chapter 1, Part 3, 3.33 Status of Foods Containing Added Coumarin. Fed. Regist. 1954, 19 (44), 1239–1240. https://www.govinfo.gov/content/pkg/FR-1954-03-05/pdf/FR-1954-03-05.pdf
5. Harmful and Potentially Harmful Constituents (HPHCs) in Tobacco Products and Tobacco Smoke: Established list. U.S. Food and Drug Administration (FDA), 2012. https://www.fda.gov/tobacco-products/rules-regulations-and-guidance/harmful-and-potentially-harmful-constituents-tobacco-products-and-tobacco-smoke-established-list