Assessment of indoor and outdoor airborne fungi in an Educational, Research and Treatment Center

Abstract

Hospital environments contain different types of microorganisms. Airborne fungal are one of these microbes and the major sources of hospitals indoor contamination that will be able to cause airborne fungal diseases. The total count and diversity of the airborne filamentous and yeasts fungi were investigated in indoor and outdoor air of selective wards of Emam Reza Educational, Research and Treatment Center. This cross-sectional study was performed during the fall season. One hundred and ninety-two environmental samples of indoor and outdoor air from hematology, infectious diseases, Ear, Nose and Throat (ENT) and Neonatal Intensive Care Unit (NICU) wards were collected by open plate technique (on Sabouraud dextrose agar media) once a week. The cultures were then examined and evaluated according to macroscopic and microscopic examination criteria. In this study, 67 (62.03%) of indoor samples and 81 (96.42%) of outdoor samples were positive for fungi. The most isolated fungi were yeast species (17.12%), <em>Penicillium</em> spp. (16.34%), <em>Alternaria</em> spp. (14.39%), <em>A.</em> <em>niger</em> (11.28%), <em>A</em>. <em>flavus</em> (8.95%), respectively. Almost all of the wards showed high rates of contamination by various fungi. However, the analysis of the data showed that indoor air of hematology ward had the highest fungal pollution. In contrast, the outdoor air of ENT had the highest fungal pollution. Thus, these results demonstrated that the cleansing and disinfection procedures in the hospital wards should be improved yet.