MULTIFUNCTIONAL HYDROXYCAMPTOTHECIN-CAPPED Fe3O4 NANOPARTICLES FOR INHIBITING OF CANCER DRUG RESISTANCE

Abstract

In this study, we have explored the multifunctional hydroxycamptothecin (HCPT)-capped Fe 3 O 4 nanoparticles efficiently targeting adriamycin-resistant K562 cells (KA cells), inducing apoptosis, making hole on KA cell membrane, photothermal therapy and low hemolysis activity. The HCPT functionalized Fe 3 O 4 nanoparticles were characterized by the transmission electron microscopy (TEM) and high performance liquid chromatographic (HPLC) study. Meanwhile, the apoptotic staining and the DNA fragmentation clearly illustrate that the apoptosis rate apparently increased after the treatment with HCPT-capped Fe 3 O 4 (HCPT- Fe 3 O 4) nanoparticles, compared with that of the only HCPT treatment in KA cells. Moreover, our observations also demonstrate the possibility of the photothermal therapy to kill KA cells by using HCPT- Fe 3 O 4 nanoparticles with semiconductor laser. In addition, the blood compatibility of the HCPT- Fe 3 O 4 nanoparticles was tested by hemolysis assay on washing the isolated rat erythrocytes. The results indicate that HCPT- Fe 3 O 4 nanoparticles did not cause aggregation behavior at lower concentration. Thus, it is evident that the HCPT functionalized Fe 3 O 4 nanoparticles could readily induce cellular apoptosis and low hemolysis activity, which could be further utilized for the efficient multimode therapy of cancers.