Fc-DIRECTED ANTIBODY CONJUGATION OF MAGNETIC NANOPARTICLES FOR ENHANCED MOLECULAR TARGETING-Reference-Cited by-同舟云学术

Fc-DIRECTED ANTIBODY CONJUGATION OF MAGNETIC NANOPARTICLES FOR ENHANCED MOLECULAR TARGETING

Published:2009-10 Issue:04 Volume:02 Page:387-396
ISSN:1793-5458
Container-title:Journal of Innovative Optical Health Sciences
language:en
Short-container-title:J. Innov. Opt. Health Sci.

Author:

REZAEIPOOR ROBABEH¹,JOHN RENU¹,ADIE STEVEN G.¹,CHANEY ERIC J.¹,MARJANOVIC MARINA¹,OLDENBURG AMY L.¹,RINNE STEPHANIE A.¹,BOPPART STEPHEN A.¹²

Affiliation:

1. Biophotonics Imaging Laboratory, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL, USA

2. Departments of Electrical and Computer Engineering, Bioengineering, and Internal Medicine, University of Illinois at Urbana-Champaign, Urbana, IL, USA

Abstract

In this study, we report the fabrication of engineered iron oxide magnetic nanoparticles (MNPs) functionalized with anti-human epidermal growth factor receptor type 2 (HER2) antibody to target the tumor antigen HER2. The Fc-directed conjugation of antibodies to the MNPs aids their efficient immunospecific targeting through free Fab portions. The directional specificity of conjugation was verified on a macrophage cell line. Immunofluorescence studies on macrophages treated with functionalized MNPs and free anti-HER2 antibody revealed that the antibody molecules bind to the MNPs predominantly through their Fc portion. Different cell lines with different HER2 expression levels were used to test the specificity of our functionalized nanoprobe for molecular targeting applications. The results of cell line targeting demonstrate that these engineered MNPs are able to differentiate between cell lines with different levels of HER2 expression.

Publisher

World Scientific Pub Co Pte Lt

Subject

Biomedical Engineering,Atomic and Molecular Physics, and Optics,Medicine (miscellaneous),Electronic, Optical and Magnetic Materials

Link

https://www.worldscientific.com/doi/pdf/10.1142/S1793545809000693

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