Pheno- and Genotypical Features of Non-Toxigenic Strains of Cholera Vibrios of Different Origins, Isolated in the Territory of Russia-Reference-Cited by-同舟云学术

Pheno- and Genotypical Features of Non-Toxigenic Strains of Cholera Vibrios of Different Origins, Isolated in the Territory of Russia

Published:2020-10-22 Issue:3 Volume: Page:89-96
ISSN:2658-719X
Container-title:Problems of Particularly Dangerous Infections
language:
Short-container-title:Problemy Osobo Opasnykh Infektsii

Author:

Levchenkо D. A.¹^ORCID,Kruglikov V. D.¹^ORCID,Gaevskaya N. E.¹^ORCID,Vodop’yanov A. S.¹,Nepomnyashchaya N. V.¹^ORCID

Affiliation:

1. Rostov-on-Don Research Anti-Plague Institute

Abstract

Aim. Analysis of the phenotypic characteristics and identification of peculiarities of the genotypic organization in non-toxigenic strains of cholera vibrios having different origin, isolated in Russia. Materials and methods. A sample of 548 non-toxigenic strains obtained using the author’s updated GIS “Cholera 1989–2014” was used. PCR genotyping was carried out in accordance with the patented “Method for the identification of non-toxigenic strains of cholera vibrio O1 serogroup using PCR to isolate genetic determinants.” Cluster analysis was performed applying the UPGMA method. The dendrogram was constructed using MEGA 5 software package.Results and discussion. Representative cultural-morphological, serological and biochemical properties of V. cholerae strains have been specified. The variability of the studied strains on the basis of phagolizability has been revealed. Unique phage-types not previously encountered in Russia have been identified. The population of non-toxigenic strains of cholera vibrio O139 serogroup is genetically homogeneous in contrast to V. cholerae O1 El Tor isolates and has identical PCR genotypes. The universality of the PCR genotyping by 14 target genes has been shown to differentiate the studied strains of V. cholerae O1 and O139, as well as to identify disparities among O139 strains isolated in different geographical regions of the country.

Publisher

Russian Research Anti-Plague Institute Microbe

Subject

Infectious Diseases,Microbiology (medical),Immunology,Microbiology,Epidemiology

Reference19 articles.

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